HuCAL VH Germline Consensus Sequences

click on any of the shaded areas to see an alignment of the germline sequences represented by the cluster to the corresponding HuCAL consensus sequence

Coverage of human VH germline sequence space by HuCAL sequences

The protein sequences representing the human VL and VH germlines were taken from VBase and aligned to the 14 HuCAL sequences. The Phylip and ClustalW (Higgins et al., 1994) phylogeny program packages were used to generate separate rootless trees for the VL kappa, VL lambda and VH sequences.

Percentages indicate the fraction of rearranged sequences in the Kabat database which cluster with the different germline subgroups. For this calculations, we used a database of rearranged sequences with 937 VL kappa, 421 VL lambda and 1267 VH sequence entries.

The VH7 sequences were included in the VH1 germline cluster