Critical Points for antibody scFV stability and folding efficency

Comparison of the thermodynamic stability and folding efficiency of the human germline consensus frameworks

Gln L6 and Gln/Glu H6: primer-induced changes of the N-terminal sequence

Core packing

Disulfide bonds and core tryptophan

Buried hydrophilic residues

Buried charge cluster (L77/L100 resp. H77/H100)

Residues with distinct conformational preferences:

Positions with conserved positive Phi torsion angles

Conserved Proline positions

Solvent exposed hydrophobic residues:

Contribution of domain interfaces to stability

The V/C interface

The Dimer interface

Problem spots by domain