Binding Affinities of recombinant Protein L, Protein LA, Protein A, and Protein G Immunoglobulin binding domains
|
| |
Protein
L*
|
Protein
A
|
Protein G
|
| Human
IgG1 |
++++ |
++++ |
++++ |
| Human
IgG2 |
++++ |
++++ |
++++ |
| Human
IgG3 |
++++ |
- |
++++ |
| Human
IgG4 |
++++ |
++++ |
++++ |
| Human
IgGA |
++++ |
++ |
- |
| Human
IgGD |
++++ |
++ |
- |
| Human
IgGE |
++++ |
++ |
- |
| Human
IgGM |
++++ |
++ |
- |
| Mouse
IgG1 |
++++ |
+ |
++ |
| Mouse
IgG2a |
++++ |
++++ |
++++ |
| Mouse
IgG2b |
++++ |
+++ |
+++ |
| Mouse
IgG3 |
++++ |
++ |
+++ |
| Mouse
IgGM |
++ |
+/- |
- |
| Rat
IgG1 |
++++ |
- |
+ |
| Rat
IgG2a |
++++ |
- |
++++ |
| Rat
IgG2b |
+++++ |
- |
++ |
| Rat
IgG2c |
++++ |
+ |
++ |
| Rat
IgGM |
+ |
+/- |
- |
| Rabbit
Ig |
+ |
++++ |
+++ |
| Hamster
Ig |
++++ |
+ |
++ |
| Guinea Pig
Ig |
++ |
++++ |
++ |
| Bovine
Ig |
- |
++ |
++++ |
| Sheep
Ig |
- |
+/- |
++ |
| Goat
Ig |
- |
+/- |
++ |
| Pig Ig |
++++ |
+++ |
+++ |
| Chicken
Ig |
++ |
- |
+ |
| Fragments |
| Human
Fab |
++++ |
+ |
+ |
| Human
F(ab')2 |
++++ |
+ |
+ |
| Human
scFv |
++++ |
+ |
- |
| Human
Fc |
- |
++ |
++ |
| Human k |
++++ |
- |
- |
| Human l |
- |
- |
- |
*It should
be noted that rProtein L binding is restricted to specific
subclasses of the k domain. Thus
the affinity indicated in the table is not general for the IgG
subclass but accounts only for those antibodies carrying the right
kappa light chain (see below). |
| Binding
of protein L to k light chains |
|
Protein L Binding |
| Human
kappa I |
++++ |
| Human
kappa II |
- |
| Human
kappa III |
++++ |
| Human
kappa IV |
++++ |
| Mouse
kappa I |
++++ |
| Mouse
kappa II |
- |
| Mouse
kappa V |
+ |
|
|
|
