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ATP Synthase
ATP synthase is one of the wonders of the molecular world. ATP synthase is an enzyme, a molecular motor, an ion pump, and another molecular motor all wrapped together in one amazing nanoscale machine. It plays an indispensable role in our cells, building most of the ATP that powers our cellular processes. The mechanism by which it performs this task is a real surprise.
Rotary Motors
ATP synthesis is composed of two rotary motors, each powered by a different fuel. The motor at the top, termed F0, an electric motor. It is embedded in the cell membrane (shown schematically as a gray stripe here), and is powered by the flow of hydrogen ions across the membrane. As the protons flow through the motor, they turn a circular rotor (shown in blue). This rotor is connected to the second motor, termed F1. The F1 motor is a chemical motor, powered by ATP. The two motors are connected together by a stator, shown on the right, so that when F0 turns, F1 turns too.Motor to Generator
So why have two motors connected together? The trick is that one motor can force the other motor to turn, and in this way, change the motor into a generator. This is what happens in our cells: the F0 motor uses the power from a proton gradient to force the F1 motor to generate ATP. In our cells, food is broken down and used to create a large excess of hydrogen ions outside the cell. The F0 portion of ATP synthase allows these ions to reenter the cell, turning the rotor in the process. As the rotor turns, it turns the axle and the F1 motor becomes a generator, creating ATP as it turns.Parts List
Large, complex molecular machines like ATP synthase pose difficult problems for structural scientists, so the structures of these machines are often determined in parts. The picture shown here is a composite of four different structures, combining structures determined by X-ray crystallography and NMR spectroscopy. The F0 motor is included in PDB file 1c17. The F1 motor and the axle that connects the two are included in PDB file 1e79. The stator has proven to be the most elusive part--the two pieces shown here are from PDB files 2a7u and 1l2p.For information on ATP synthase from a genomics perspective, take a look at the Protein of the Month at the European Bioinformatics Institute.
Next: Exploring the F1 Structure
Last changed by: A.Honegger,